O-tert-Butyl-L-threonine is an amino acid derivative belonging to the class of threonine analogs. As a modified amino acid, it retains the side chain's hydroxyl functionality, capped by a tert-butyl group that offers enhanced chemical stability and hydrophobic properties. This modification plays a critical role in peptide synthesis, where it can be utilized to protect hydroxyl groups during stepwise chain elongation. Furthermore, the compound is valuable in probing protein structures due to its steric bulk, which affects protein folding and interactions, facilitating advanced protein engineering studies.